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  • Title: Quantum dot-based mortalin staining as a visual assay for detection of induced senescence in cancer cells.
    Author: Kaul Z, Yaguchi T, Chiura HX, Kaul SC, Wadhwa R.
    Journal: Ann N Y Acad Sci; 2007 Apr; 1100():368-72. PubMed ID: 17460200.
    Abstract:
    Quantum dots (QDs) are fluorescent nanocrystals that are emerging as fine alternatives to the conventional organic dyes. They have several advantages including greater photostability and a wider range of excitation-emission wavelengths. By using mortalin staining as a model, we initially demonstrated that the QDs are more stable and provide better resolution in protein imaging in fixed cells. With the help of an internalizing antibody, we generated internalizing QD (i-QD) and demonstrated its inertness to cell replication, structure, and viability. Based on the superior resolution, stability and inertness, we propose the use of QD staining of mortalin as a cell-based visual assay to screen for senescence-inducing drugs, proteins, and siRNAs.
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