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  • Title: Quantitative kinetic analysis in a microfluidic device using frequency-domain fluorescence lifetime imaging.
    Author: Matthews SM, Elder AD, Yunus K, Kaminski CF, Brennan CM, Fisher AC.
    Journal: Anal Chem; 2007 Jun 01; 79(11):4101-9. PubMed ID: 17472341.
    Abstract:
    A novel microfluidic approach for the quantification of reaction kinetics is presented. A three-dimensional finite difference numerical simulation was developed in order to extract quantitative kinetic information from fluorescence lifetime imaging experimental data. This approach was first utilized for the study of a fluorescence quenching reaction within a microchannel; the lifetime of a fluorophore was used to map the diffusion of a quencher across the microchannel. The approach was then applied to a more complex chemical reaction between a fluorescent amine and an acid chloride, via numerical simulation the bimolecular rate constant for this reaction was obtained.
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