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  • Title: Twisted or shifted? Fluorescence measurements of late intermediates in transcription initiation by T7 RNA polymerase.
    Author: Turingan RS, Theis K, Martin CT.
    Journal: Biochemistry; 2007 May 29; 46(21):6165-8. PubMed ID: 17472344.
    Abstract:
    T7 RNA polymerase undergoes dramatic structural rearrangements in the transition from initiation to elongation. Two models have been proposed for promoter-bound intermediates late in the transition. (i) A subset of promoter interactions are maintained through completion of the protein conformational (twist) change, and (ii) concerted movement (shift) of all promoter-binding elements away from the growing DNA-RNA hybrid leads to an open intermediate, with large-scale domain rotations deferred until after promoter release. Fluorescence resonance energy transfer measurements provide very strong support for the latter.
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