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  • Title: [Immunocytochemical analysis of interleukin-1 beta production by human monocytes].
    Author: Simbirtsev AS, Konusova VG, Ketlinskiĭ SA.
    Journal: Biull Eksp Biol Med; 1991 Sep; 112(9):278-80. PubMed ID: 1747477.
    Abstract:
    IL-1 was localized within the cytoplasm of human blood monocytes by indirect immunofluorescence using polyclonal rabbit antibodies. After LPS stimulation first IL-1-positive cells appeared at 2 hours and maximal intracellular IL-1 concentration was observed at 10-24 hours when nearly 90% monocytes were labeled with subsequent decline at 48 hours. The highest intracellular IL-1 content preceded its maximal level in cell supernatants.
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