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  • Title: Expression of human acidic fibroblast growth factor in Nicotiana benthamiana with a potato-virus-X-based binary vector.
    Author: Liu J, Ma P, Sun Y, Yang M, Yang L, Li Y, Wu Y, Zhu X, Wang X.
    Journal: Biotechnol Appl Biochem; 2007 Nov; 48(Pt 3):143-7. PubMed ID: 17484724.
    Abstract:
    The aFGF (acidic fibroblast growth factor) plays an important role in morphogenesis, angiogenesis and wound healing and is therefore of potential medical interest. A DNA fragment encoding haFGF (human aFGF) has been cloned into the PVX (potato virus X)-based binary vector (pgR107) and transiently expressed in Nicotiana benthamiana (a wild Australian tobacco) by agroinfection. Approx. 1 week after agroinfection, the recombinant haFGF accumulated in the agroinfected plants reached up to 1% of the total soluble protein. haFGF was then purified on heparin-Sepharose CL-6B. The purified haFGF could stimulate the growth of NIH 3T3 cells, suggesting that the recombinant haFGF expressed via PVX viral vector in N. benthamiana was active biologically.
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