These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Molecular characterization of an epithelial Ca2+ channel-like gene from crayfish Procambarus clarkii.
    Author: Gao Y, Wheatly MG.
    Journal: J Exp Biol; 2007 May; 210(Pt 10):1813-24. PubMed ID: 17488945.
    Abstract:
    This study describes the cloning, sequencing and functional characterization of an epithelial Ca(2+) channel (ECaC)-like gene isolated from antennal gland (kidney) of the freshwater crayfish Procambarus clarkii. The full-length cDNA consisted of 2687 bp with an open reading frame of 2169 bp encoding a protein of 722 amino acids with a predicted molecular mass of 81.7 kDa. Crayfish ECaC had 76-78% identity at the mRNA level (80-82% amino acid identity) with published fish sequences and 56-62% identity at the mRNA level (52-60% amino acid identity) with mammalian ECaCs. Secondary structure of the crayfish ECaC closely resembled that of cloned ECaCs. Postmolt ECaC expression was exclusively restricted to epithelia associated with Ca(2+) influx and was virtually undetectable in non-epithelial tissues (eggs, muscle). Compared with expression levels in hepatopancreas, expression in gill was 10-fold greater and expression was highest in antennal gland (15-fold greater than in hepatopancreas). Compared with baseline expression levels in intermolt stage, expression of ECaC in antennal gland increased 7.4- and 23.8-fold, respectively, in pre- and postmolt stages of the molting cycle. This increase was localized primarily in the labyrinth and nephridial canal, regions of the antennal gland associated with renal Ca(2+) reabsorption. The ECaC in crayfish appears to be expressed in epithelia associated with unidirectional Ca(2+) influx and relative expression is correlated with rate of Ca(2+) influx.
    [Abstract] [Full Text] [Related] [New Search]