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  • Title: A high-performance liquid chromatography with UV-vis detection method for the determination of Brazilein in plant extract.
    Author: Yan X, Wang W, Zhang L, Zhao Y, Xing D, Du L.
    Journal: J Chromatogr Sci; 2007 Apr; 45(4):212-5. PubMed ID: 17504570.
    Abstract:
    A previously established mobile phase composition for a high-performance liquid chromatography (HPLC)-electrochemical detection (ED) method for the determination of Brazilein is further examined by HPLC-UV. Each component in the mobile phase is evaluated by the HPLC-UV system, and a convenient HPLC-UV method for the determination of Brazilein is developed. The mobile phase of this HPLC-UV method consists of sodium dihydrogen phosphate (NaH(2)PO(4)). The detection limit of Brazilein (signal-to-noise = 3) is 1 ng, and the calibration graph of Brazilein ranges from 1-120 ng per 20-microL injection. The validity of the proposed HPLC-UV method is demonstrated by the analysis of Brazilein in real plant samples. The differences between the HPLC-ED method and the proposed HPLC-UV method are given.
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