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  • Title: Production of Fusarium solani f. sp. pisi cutinase in Fusarium venenatum A3/5.
    Author: Sørensen JD, Petersen EI, Wiebe MG.
    Journal: Biotechnol Lett; 2007 Aug; 29(8):1227-32. PubMed ID: 17505784.
    Abstract:
    Fusarium venenatum A3/5 was transformed using the Aspergillus niger expression plasmid, pIGF, in which the coding sequence for the F. solani f. sp. pisi cutinase gene had been inserted in frame, with a KEX2 cleavage site, with the truncated A. niger glucoamylase gene under control of the A. niger glucoamylase promoter. The transformant produced up to 21 U cutinase l(-1) in minimal medium containing glucose or starch as the primary carbon source. Glucoamylase (165 U l(-1) or 8 mg l(-1)) was also produced. Both the transformant and the parent strain produced cutinase in medium containing cutin.
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