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  • Title: Evaluation of an automated soluble transferrin receptor (sTfR) assay on the Roche Hitachi analyzer and its comparison to two ELISA assays.
    Author: Pfeiffer CM, Cook JD, Mei Z, Cogswell ME, Looker AC, Lacher DA.
    Journal: Clin Chim Acta; 2007 Jul; 382(1-2):112-6. PubMed ID: 17511979.
    Abstract:
    BACKGROUND: Soluble transferrin receptor (sTfR) assays are currently not standardized. This hinders data comparison between studies and also affects the use of a recently proposed model to estimate body iron. METHODS: We evaluated the analytical performance of a fully automated sTfR immunoturbidimetric assay (Roche Diagnostics) and compared it with two ELISA assays (Ramco Laboratories and an in-house ELISA assay used in the body iron model). RESULTS: The Roche assay showed excellent intra- and inter-assay precision (CV<5%). Prolonged exposure of serum samples to room temperature and multiple freeze-thaw cycles did not affect sTfR concentrations. Receiver-operator characteristic curve analysis demonstrated that the Roche assay (area-under-the-curve (AUC)=0.882) was superior to the Ramco assay (AUC=0.794) in predicting iron deficiency (defined as serum ferritin <10 microg/L; P=0.013). Method comparison between the Roche and the two ELISA assays showed good correlations (r>0.8); however, sTfR values by the Roche assay were on average 30% lower than values obtained with the two ELISA assays. CONCLUSIONS: sTfR data measured with an immunoturbidimetric assay can be compared to a commonly used ELISA assay, and can be used in the body iron model through regression equations obtained in the present study.
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