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  • Title: Rapid, simple and effective technical procedure for the regeneration of IgG and HSA affinity columns for proteomic analysis.
    Author: Millioni R, Puricelli L, Iori E, Tessari P.
    Journal: Amino Acids; 2008 Apr; 34(3):507-9. PubMed ID: 17514490.
    Abstract:
    In plasma and serum, the presence of high-abundance proteins can overwhelm the signals of low-abundance proteins, which then become undetectable either by two-dimensional gels or chromatographic techniques. Therefore, depletion of abundant proteins is a prerequisite to detect low-abundance components. Furthermore, the regeneration of pre-purification tools could be money-saving. We applied an affinity chromatography kit to remove albumin and the immunoglobulin chains from plasma and propose a simple and effective technical procedure for the regeneration of these affinity columns.
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