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  • Title: Characterization of two temperature-inducible promoters newly isolated from B. subtilis.
    Author: Li W, Li HX, Ji SY, Li S, Gong YS, Yang MM, Chen YL.
    Journal: Biochem Biophys Res Commun; 2007 Jul 13; 358(4):1148-53. PubMed ID: 17521615.
    Abstract:
    Here, two temperature sensitive promoters, P2 and P7, isolated from Bacillus subtilis, were characterized. The production of beta-galactosidase driven by these promoters was much higher at 45 degrees C than that at 37 degrees C both in Escherichia coli and B. subtilis and that the P2 promoter showed higher expression strength in B. subtilis at 45 degrees C. Thereby, an efficient temperature-inducible expression system was constructed by using P2 promoter in B. subtilis. Thus, we isolated and characterized a newly temperature inducible promoter and exploited it as a potential expression element in B. subtilis.
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