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  • Title: Mixed-species biofilm formation by lactic acid bacteria and rice wine yeasts.
    Author: Kawarai T, Furukawa S, Ogihara H, Yamasaki M.
    Journal: Appl Environ Microbiol; 2007 Jul; 73(14):4673-6. PubMed ID: 17526778.
    Abstract:
    We found that species combinations such as Lactobacillus casei subsp. rhamnosus IFO3831 and Saccharomyces cerevisiae Kyokai-10 can form a mixed-species biofilm in coculture. Moreover, the Kyokai-10 yeast strain can form a biofilm in monoculture in the presence of conditioned medium (CM) from L. casei IFO3831. The active substance(s) in bacterial CM is heat sensitive and has a molecular mass of between 3 and 5 kDa. In biofilms from cocultures or CM monocultures, yeast cells had a distinct morphology, with many hill-like protrusions on the cell surface.
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