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  • Title: IL-4 gene transcription in human T cells is suppressed by T-bet.
    Author: Kitamura N, Kitamura F, Kaminuma O, Miyatake S, Tatsumi H, Nemoto S, Mori A.
    Journal: Int Arch Allergy Immunol; 2007; 143 Suppl 1():68-70. PubMed ID: 17541280.
    Abstract:
    UNLABELLED: T-bet is crucially implicated in Th1 differentiation due to its strong promoting activity for IFN-gamma gene transcription. However, the regulatory role of T-bet in Th2 cytokines is not fully delineated. METHODS: The effect of T-bet on mRNA expression as well as the promoter activity of IL-4 in human T cells was investigated by employing quantitative RT-PCR and fluorescence-based promoter reporter assay procedures. RESULTS: IL-4 mRNA expression as well as the transcriptional activity of 5'-flanking region in the IL-4 gene encompassing -1105 to +4 in Jurkat cells was clearly upregulated upon stimulation. The inducible mRNA expression and the promoter activity of IL-4 were significantly diminished by ectopic expression of T-bet. CONCLUSION: IL-4 gene transcription is inhibited by T-bet via the suppression of its promoter activity, independently of IFN-gamma. T-bet facilitates Th1 differentiation through not only upregulation of IFN-gamma, but also downregulation of IL-4 gene transcription.
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