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  • Title: Improvement of the multiple-stress tolerance of an ethanologenic Saccharomyces cerevisiae strain by freeze-thaw treatment.
    Author: Wei P, Li Z, Lin Y, He P, Jiang N.
    Journal: Biotechnol Lett; 2007 Oct; 29(10):1501-8. PubMed ID: 17541503.
    Abstract:
    An effective, simple, and convenient method to improve yeast's multiple-stress tolerance, and ethanol production was developed. After an ethanologenic Saccharomyces cerevisiae strain SC521 was treated by nine cycles of freeze-thaw, a mutant FT9-11 strain with higher multiple-stress tolerance was isolated, whose viabilities under acetic acid, ethanol, freeze-thaw, H(2)O(2), and heat-shock stresses were, respectively, 23-, 26-, 10- and 7-fold more than the parent strain at an initial value 2 x 10(7) c.f.u. per ml. Ethanol production of FT9-11 was similar (91.5 g ethanol l(-1)) to SC521 at 30 degrees C with 200 g glucose l(-1), and was better than the parent strain at 37 degrees C (72.5 g ethanol l(-1)), with 300 (111 g ethanol l(-1)) or with 400 (85 g ethanol l(-1)) g glucose l(-1).
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