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  • Title: Characterisation of levofloxacin-resistant clinical isolates of Streptococcus pyogenes in Bilbao, Spain.
    Author: Alonso R, Mateo E, Ezpeleta G, Cisterna R.
    Journal: Int J Antimicrob Agents; 2007 Aug; 30(2):183-5. PubMed ID: 17543504.
    Abstract:
    The resistance mechanisms and clonal relationship were determined for two Streptococcus pyogenes isolates with high-level resistance to levofloxacin as well as to other fluoroquinolones. DNA amplification and sequencing revealed a serine-81-->phenylalanine substitution in GyrA and a double substitution in ParC of serine-79-->phenylalanine and aspartic acid-91-->asparagine. Pulsed-field gel electrophoresis analysis and emm typing determined that both isolates were emm type 28 and were genetically indistinguishable.
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