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  • Title: Production of a Rhizopus oryzae lipase from Pichia pastoris using alternative operational strategies.
    Author: Surribas A, Stahn R, Montesinos JL, Enfors SO, Valero F, Jahic M.
    Journal: J Biotechnol; 2007 Jun 30; 130(3):291-9. PubMed ID: 17544535.
    Abstract:
    Different cultivation strategies have been compared for the production of Rhizopus oryzae lipase (ROL) from Pichia pastoris. Several drawbacks have been found using a methanol non-limited fed-batch. On the one hand, oxygen limitation appeared at early cell dry weights and, on the other hand, high cell death was observed. A temperature limited fed-batch has been proposed to solve both problems. However, in our case study a methanol non-limited fed-batch results in better productivities. Finally, a lower salt medium were used to overcome cell death problems and a temperature limited fed-batch was applied thereafter to solve oxygen transfer limitations. This combined strategy has resulted in lower productivities when compared to a methanol non-limited fed-batch. However the culture could be longer prolonged and a 1.3-fold purer final product was obtained mainly due to cell death reduction.
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