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  • Title: Maternal transmission of Wolbachia in Phlebotomus papatasi (Scopoli).
    Author: Kassem HA, Osman G.
    Journal: Ann Trop Med Parasitol; 2007 Jul; 101(5):435-40. PubMed ID: 17550649.
    Abstract:
    Attempts have been made to transfer Wolbachia from infected to uninfected, laboratory-reared Phlebotomus papatasi, through mating, and to determine whether the incompatibility phenotype could be expressed through crosses between infected and uninfected flies. In order to test for the intraspecific transmission of Wolbachia in crosses between infected females and uninfected males, or those between uninfected females and infected males, a PCR based on Wolbachia -specific wsp primers was used to test the progeny of each cross and, subsequently, 50 individual flies from the F(3) generation. All the individual flies tested from the F(1) progeny of the crosses between infected males and uninfected females were found to be uninfected. In the crosses involving infected females and uninfected males, however, Wolbachia were found in the progeny of five matings out of the 23 that produced viable eggs. In the F(3), Wolbachia were not detected in any of the individuals resulting from the cross between uninfected females and infected males but they were detected in 52% (26) of the 50 tested individuals resulting from the cross between infected females and uninfected males. No evidence of cytoplasmic incompatibility (CI) was observed in any of the crosses. The absence of CI expression and relatively low frequencies of maternal transmission could hamper the potential use of Wolbachia in a transgenic strategy for the control of leishmaniases.
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