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  • Title: [Determination of aisaticoside in Centella asiatica extract by HPLC-ELSD].
    Author: Yu QL, Gao WY, Chen HX, Duan HQ.
    Journal: Zhongguo Zhong Yao Za Zhi; 2007 Mar; 32(6):503-5. PubMed ID: 17552155.
    Abstract:
    OBJECTIVE: A HPLC-ELSD method was established for the simultaneous quantitative determination of asiaticoside in Centella asiatica extract. METHOD: The column was packed with 5 m HIQ C18 stationary phase. The mobile phase consisted of acetonitrile-water, eluted in gradient mode. The temperature of drift tube was 105 degrees C and the nebulizer nitrogen flow rate was 2.9 L min(-1). RESULT: The linear ranges of the asiaticoside were 0.35-7.0 microg. The recovery of the asiaticoside in C. asiatica extract was 94.9% (RSD 1.7%). CONCLUSION: The method is reliable, simple, precise and could be used for the quality control of C. asiatica extract.
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