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  • Title: Involvement of protein kinase A in nitric oxide stimulating effect on a BK(Ca) channel of human dermal fibroblasts.
    Author: Roh S, Choi S, Lim I.
    Journal: J Invest Dermatol; 2007 Nov; 127(11):2533-8. PubMed ID: 17554366.
    Abstract:
    We reported previously that a large-conductance Ca2+-activated K+ (BK(Ca)) channel constitutes a significant fraction of the K+ current in human dermal fibroblasts, and that nitric oxide (NO) increases the open-channel probability (NPo) of BK(Ca) channels via a soluble guanylate cyclase (sGC)/cyclic guanosine monophosphate (cGMP)/protein kinase G (PKG) pathway. The purpose of this study was to investigate whether the adenylate cyclase (AC)/cAMP/protein kinase A (PKA) pathway may also be involved in NO action on BK(Ca) channels in human dermal fibroblasts. Electrophysiological single-channel recordings were performed on fifth-passage cells of human penile skin cultures. KT5720 (specific PKA inhibitor) blocked the stimulatory effect of sodium nitroprusside (NO donor) on BK(Ca) channels. By contrast, forskolin (AC activator) or 8-bromo-cAMP (cell-permeable cAMP analog) did not increase the NPo of the channel. The PKA catalytic subunit (PKAcs) alone did not increase the NPo of the channel in cell-attached and inside-out patches, however, PKAcs with cGMP increased the NPo. In contrast, PKAcs with cGMP did not increase the NPo of BK(Ca) channels with 1H-[1,2,4]-oxadiazolo[4,3-a]quinoxalin-1-one pretreatment, and KT5720 pretreatment also blocked the stimulatory effect of 8-Br-cGMP. In conclusion, the present data suggest the involvement of PKA in the stimulatory effect of NO on the BK(Ca) channel in human dermal fibroblasts through cGMP.
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