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  • Title: [Experimental study on transplantation of embryonic stem cells in treating spinal cord injury].
    Author: Yang J, Li C, Zhai R.
    Journal: Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi; 2007 May; 21(5):487-91. PubMed ID: 17578288.
    Abstract:
    OBJECTIVE: To observe the effect of transplantation of embryonic stem cell (ES) on neurological functional recovery of injured spinal cord in adult mouse. METHODS: The ES cells were cultured and induced in vitro. Fifty C57/BL6J mice were made animal model of semi-cut mice of T9,10. The ES cell-derived neural precursors cells were transplanted into the vertebral canal around injured spinal cord semi-cut mice. Twenty-eight C57/BL6J mice were randomly divided into three groups: sham operation group(group A, n=9), operation/cell group (group B, n = 10), and operation/DMEM group (group C, n = 9). RT-PCR analysis, X-gal staining and immunofluorescence were used to observe the cells survival and differentiation in the spinal crod. BBB test was performed to study functional improvement. RESULTS: ES cells induced and cultured in vitro displayed clonal growth with circle or ovoid shape and had one or more nucleoli. RT-PCR result showed that the induced ES cells expressed mRNA of Nestin and microtubule-associated protein, but did not express glial fibrillory acidic protein (GFAP). There was statistically significant difference in BBB scoring between group A and groups B, C after operation (P <0.01). There was statistically significant difference in BBB scoring at 1, 2 and 4 weeks of operation (P < 0.01), but no statistically significant difference at 6 and 8 weeks of operation between groups B and C (P>0.05). The X-gal staining results were positive in group B and negative in groups A and C. The immunoflurescence result showed neurofilament green fluor and no expression of GFAP in injured spinal cord region. CONCLUSION: After transplantation, ES cell-derived cells can survive, transfer into the injury position, and differentiate into neurons, but spinal cord function has no obvious improvement.
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