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  • Title: Suppression of C-hordein synthesis in barley by antisense constructs results in a more balanced amino acid composition.
    Author: Lange M, Vincze E, Wieser H, Schjoerring JK, Holm PB.
    Journal: J Agric Food Chem; 2007 Jul 25; 55(15):6074-81. PubMed ID: 17580876.
    Abstract:
    Barley has for feeding purposes a shortage of essential amino acids, especially lysine, threonine, and methionine, and an excess of proline and glutamine. In the present study, we have introduced into barley an antisense construct against C-hordeins, the storage protein with the lowest nutritional quality. SDS-PAGE and reverse phase HPLC revealed a relative reduction in the amounts of C-hordeins and relative increases in the content of the other storage proteins. The five different lines analyzed had lower amounts of proline, glutamic acid/glutamine, and phenylalanine (up to 12%, 6%, and 9% reductions), while the lysine, threonine, and methionine content was increased with up to 16%, 13% and 11%. It is concluded that antisense mediated suppression of C-hordein synthesis may be a promising approach for improving the nutritional value of barley as a feed crop while at the same time reducing the environmental nitrogen load.
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