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  • Title: Studies of the bacteriorhodopsin photocycle without the use of light: clues to proton transfer coupled reactions.
    Author: Lanyi JK.
    Journal: J Mol Microbiol Biotechnol; 2007; 12(3-4):210-7. PubMed ID: 17587869.
    Abstract:
    In the photochemical cycle of bacteriorhodopsin, the light-driven proton pump of halobacteria, only the first step, the isomerization of the all-trans retinal to 13-cis, is dependent on illumination. Because the steps that accomplish the translocation of a proton during the ensuing reaction sequence of intermediate states are thermal reactions, they have direct analogies with such steps in other ion pumps. In a surprisingly large number of cases, the reactions of the photocycle could be studied without using light. This review recounts experiments of this kind, and what they contribute to understanding the transport mechanism of this pump, and perhaps indirectly other ion pumps as well.
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