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  • Title: Designing a highly efficient refolding system for alkaline phosphatase using combination of cyclodextrin and Mg2+ ion.
    Author: Khodagholi F, Yazdanparast R.
    Journal: Protein J; 2008 Jan; 27(1):1-6. PubMed ID: 17588144.
    Abstract:
    Two different folding aids including alpha-cyclodextrin and Mg2+ ions were applied to alkaline phosphatase refolding. The refolding yield depending on concentration of each of the refolding agents reached to 55 and 52% in the presence of 100 mM alpha-cyclodextrin and/or 5 mM Mg2+ ions, respectively. However, the refolding yield, mediated by combination of alpha-cyclodextrin and Mg2+ ions, was more than 96%. Replacement of Mg2+ ion with other type of ions which interact with alpha-cyclodextrin interfere with the function of cyclodextrin resulting in low refolding yields.
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