These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


PUBMED FOR HANDHELDS

Search MEDLINE/PubMed


  • Title: [Effects of fluid shear stress on the expression of IL-8 receptor mRNA in EA. Hy926 cells].
    Author: Yu C, Liu X, Zhang Y.
    Journal: Sheng Wu Yi Xue Gong Cheng Xue Za Zhi; 2007 Apr; 24(2):303-7. PubMed ID: 17591247.
    Abstract:
    This study was conducted to elucidate the effects of different fluid shear stress on the expression of IL-8 receptors CXCR1 and CXCR2 mRNA in endothelial cells, EA. Hy926 cells. The HUVEC cell lines were cultured in vitro and then exposed to 5.56, 10.02, and 15.27 dyn/cm2 fluid shear stress respectively for 5 min, 10 min, 15 min, 20 min, 25 min, 30 min, 1 h, 2 h, 4 h and 8 h. Semi-quantitative reversal transcription-polymerase chain reaction (RT-PCR) was used for detecting IL-8 receptors mRNA expression at different times. The results showed that, under 5. 56 dyn/cm2 shear stress, both the expression of CXCR1 and CXCR2 mRNA increased significantly with time (P<0.05). When exposed to 10. 02 dyn/cm2, the expression of CXCR1 mRNA was down-regulated with time on every occasion. CXCR2 mRNA increased temporally at 30 min, then it decreased gradually with time and finally went on at a constant lower level. When exposed to 15.27 dyn/cm2, both CXCR1 and CXCR2 mRNA expression decreased significantly with time (P<0.01). These data indicate that the expression of CXCR1 and CXCR2 mRNA of endothelial cell is regulated by fluid shear stress.
    [Abstract] [Full Text] [Related] [New Search]