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  • Title: Expression analysis of extracellular matrix components in brush biopsies of oral lesions.
    Author: Driemel O, Kosmehl H, Rosenhahn J, Berndt A, Reichert TE, Zardi L, Dahse R.
    Journal: Anticancer Res; 2007; 27(3B):1565-70. PubMed ID: 17595777.
    Abstract:
    BACKGROUND: Oral brush biopsies have proved to be a promising new non-invasive methodology in the diagnosis of oral lesions. The extracellular matrix (ECM) molecules gamma2 chain of laminin-5 (L5gamma2), tenascin-c (Tn-C) and the fibronectin isoform containing EDB (EDB-fn) are involved in matrix remodeling during malignant transformation in oral carcinoma. MATERIALS AND METHODS: Expression of L5gamma2, Tn-C and EDB-fn was analysed in brush biopsy-obtained cells of benign inflammatory or hyperproliferative lesions and primary oral squamous cell carcinoma (OSCC) using the Roche LightCycler 2.0 System. RESULTS AND CONCLUSION: Oral carcinoma are detectable with mRNA resynthesis of the ECM molecules L5gamma2 and Tn-C in oral brush biopsies. EDB-fn mRNA was not detected--the stroma myofibroblasts are apparently a preferential source of EDB-fn and sampling by oral brush biopsy harvests epithelial cells and does not reach the cells which do express EDB-fn. The performance of gene expression analysis in brush biopsies is limited by a high RNase activity in the oral cavity.
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