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  • Title: Evidence for targeting common siRNA hotspots and GC preference by plant Dicer-like proteins.
    Author: Ho T, Wang H, Pallett D, Dalmay T.
    Journal: FEBS Lett; 2007 Jul 10; 581(17):3267-72. PubMed ID: 17597620.
    Abstract:
    Small interfering (si)RNAs isolated from Brassica juncea leaves infected by Turnip mosaic virus (TuMV) were characterized by cloning and sequencing. The TuMV siRNA population was dominated by 21 and 22-nt long species originated mainly from the same siRNA hotspots, indicating operational similarity between the plant Dicer-like (DCL) enzymes. Robust GC bias was observed for TuMV siRNAs versus the virus genome, indicating that DCL was more likely to target GC-rich regions. Furthermore, dicot micro-(mi)RNAs displayed higher GC% than their DCL1 substrate RNAs, implicating that the GC bias may be ancient, therefore may be important for the RNAi technology.
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