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  • Title: A calorimetric study of the Ni(II)-5' AMP system. A base-stacking stabilization.
    Author: Herrero LA, Calafat AM, Terrón A.
    Journal: Eur J Biochem; 1991 Dec 05; 202(2):401-4. PubMed ID: 1761042.
    Abstract:
    The thermodynamic parameters of the interaction between Ni(II) (M) and 5'-AMP in aqueous solution were determined calorimetrically (I = 0.1 tetramethylammonium bromide, 25 degrees C) at physiological pH. The experimental conditions were carefully selected to avoid the polynuclear complex formation. From the Ni(5'AMP)-complex-formation constant (logK1 = 2.55), the Ni(5'AMP)2(2-)-complex-formation constant and the Ni(5'AMP) (ML) and Ni(5'AMP)2(2-) (ML2) complex-formation enthalpies were determined (logK2 = 2.34, delta H1 = -10.0 kJ/mol and delta H2 = 21.6 kJ/mol). These results confirm the formation of the ML2 complex in solution and agree very well with the hypothesis of the 'stacking' between the purine rings, promoted for metal ions.
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