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  • Title: Scattering suppression and confocal detection in multifocal multiphoton microscopy.
    Author: Martini J, Andresen V, Anselmetti D.
    Journal: J Biomed Opt; 2007; 12(3):034010. PubMed ID: 17614718.
    Abstract:
    We have developed a new descanned parallel (32-fold) pinhole and photomultiplier detection array for multifocal multiphoton microscopy that effectively reduces the blurring effect originating from scattered fluorescence photons in strongly scattering biological media. With this method, we achieve a fourfold improvement in photon statistics for detecting ballistic photons and an increase in spatial resolution by 21% in the lateral and 35% in the axial direction compared to single-beam non-descanned multiphoton microscopy. The new detection concept has been applied to plant leaves and pollen grains to verify the improvements in imaging quality.
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