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  • Title: Capillary electrophoresis of DNA in entangled polymer solutions.
    Author: Grossman PD, Soane DS.
    Journal: J Chromatogr; 1991 Oct 18; 559(1-2):257-66. PubMed ID: 1761626.
    Abstract:
    Electrophoretic separations of DNA restriction fragments were performed in solutions of hydroxyethylcellulose (HEC) using capillary electrophoresis. Rheological studies confirmed that the entanglement threshold (phi*) for the solution is ca. 0.003 g/ml, in good agreement with theoretical predictions. A mesh size an order of magnitude smaller than that found in agarose gels was calculated using polymer-entanglement theory and was confirmed by electrophoretic measurements. Electrophoretic migration was shown to follow the Ogston regime under most conditions. An approach for obtaining smaller mesh sizes is presented.
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