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  • Title: Direct determination of selenium in human serum by inductively coupled plasma-collision cell-mass spectrometry.
    Author: Burri J, Haldimann M.
    Journal: Clin Chem Lab Med; 2007; 45(7):895-8. PubMed ID: 17617034.
    Abstract:
    BACKGROUND: Selenium status in humans depends on nutritional intake. To facilitate a future monitoring study with a large number of samples, an inductively coupled plasma mass spectrometry (ICP-MS) analysis method including collision cell technology was developed for the direct determination of selenium in human serum. METHODS: Serum samples were diluted (1:15 v/v) with an aqueous solution containing 2-propanol, EDTA(NH(4))(2), ammonia and Triton X-100. Isotope dilution was used as the calibration method, evaluating the (78)Se/(82)Se ratio. Interference by (81)BrH(+) was corrected mathematically. Nebuliser blockage was prevented by sample centrifugation and filtration of the wash solution. Method validation was carried out using certified and spiked human sera. RESULTS: All values obtained for the accuracy tests were either within the certified ranges or showed recovery between 100.9% and 104.7%. Further results were relative standard deviation of 2.0% for repeatability and 3.0% for between-run precision, a limit of detection of 0.5 mug/L and a limit of quantification of 1.1 microg/L. CONCLUSIONS: The benefits of the new method are high accuracy combined with low sample consumption and fast sample throughput.
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