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  • Title: [Sequence-length polymorphism research of C-stretch in mitochondrial DNA hyper-variant region].
    Author: Tuo Y, Liu QB, Li SB.
    Journal: Fa Yi Xue Za Zhi; 2007 Apr; 23(2):123-6. PubMed ID: 17619460.
    Abstract:
    OBJECTIVE: To investigate the polymorphism of C-stretch in mitochondrial DNA hyper-variant region and the values in forensic science. METHODS: The gene frequency were detected in such two loci of nt16180 and nt310 by using PCR-sequencing technique with two pair of primers according to the reference. RESULTS: The length of PCR products were 807 bp and 962 bp respectively. Seven genotypes in each locus were detected. AAAACCCCCTCCCC allele amounted to 87.72% of all nt16180 genotype, while AAAACCCCCCCCCCCCC was firstly reported in Han population. CCCCCCCCTCCCCCC allele amounted to 60.53% of all nt310 genotype. 15 haplotypes were detected among 114 samples. The GD value was 0.6309. The AAAACCCCCTCCCC-CCCCCCCCTCCCCCC haplotype was the most high frequency and amounted to 57.89%. CONCLUSION: The obtained basic genetic data in two loci of nt16180 and nt310 of mtDNA will be useful for forensic science.
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