These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Heating induces aggregation and decreases detection of serotonin transporter protein on western blots.
    Author: McLane MW, Hatzidimitriou G, Yuan J, McCann U, Ricaurte G.
    Journal: Synapse; 2007 Oct; 61(10):875-6. PubMed ID: 17623834.
    Abstract:
    In recent years, there has been growing interest in the use of Western blot analysis to monitor changes in the abundance of the serotonin transporter (SERT) protein. In the Western blot procedure, heat denaturation is a common, early step. We now report that heating samples to 90 degrees C decreases the abundance of the SERT protein band and causes dispersion of a majority of the SERT signal to a high molecular weight smear. These observations are in keeping with the fact that heating can influence the electrophoretic behavior of some proteins. By omitting the heat denaturation step in the Western blot procedure, better detection of the SERT protein is achieved.
    [Abstract] [Full Text] [Related] [New Search]