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  • Title: [Protective effect of N-acetyl-L-cysteine on liver and lung injury in mice after partial hepatic ischemia/reperfusion].
    Author: Wu HS, Jin X, Tian Y, Zhang JH, Wang CY.
    Journal: Zhongguo Wei Zhong Bing Ji Jiu Yi Xue; 2007 Jul; 19(7):394-7. PubMed ID: 17631703.
    Abstract:
    OBJECTIVE: To investigate the changes in Toll-like receptor 2/4(TLR2/4) gene expression in liver and lung in ischemia/reperfusion (I/R) injury with or without preconditioning of N-acetyl-L-cysteine (NAC). METHODS: BALB/c mice were used in a model of partial hepatic I/R injury and randomly divided into sham-injury control group (SH group), hepatic I/R group and hepatic I/R with NAC pretreatment group (NAC group), each n=10. The level of tumor necrosis factor-alpha (TNF-alpha) in portal vein and plasma alanine aminotransferase (ALT) were measured at 1 hour and 3 hours respectively after reperfusion, the expressions of TLR2/4 mRNA and protein in liver and lung were observed with reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting at the same time points. RESULTS: Compared with I/R group, the expressions of TLR2/4 mRNA and protein in liver and lung in NAC group were decreased at same time points (P<0.05 or P<0.01). The level of TNF-alpha in portal vein and plasma ALT increased continually in I/R group at 1 hour and 3 hours after reperfusion compared with SH group, however, they were significantly lowered in the group pretreated by NAC (P<0.05 or P<0.01). CONCLUSION: TLR2/4 is activated in liver and lung in the process of partial hepatic I/R injury. NAC can inhibit the activation of TLR2/4 and the induction of TNF-alpha resulted from I/R injury via modulating the state of redox process; thus it might mitigate liver and lung injury following partial hepatic I/R in mice.
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