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  • Title: [Study on the localization of a major allergen Der f 2 by fluorescence immunohistochemical method].
    Author: Li M, Bao Y, Liu ZG.
    Journal: Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi; 2007 Feb 28; 25(1):49-52. PubMed ID: 17639700.
    Abstract:
    OBJECTIVE: To localize Der f 2 in the body of Dermatophagoides farinae. METHODS: Live mites were embedded. Serial mite paraffin sections were made and checked under light microscope. The sections were incubated with anti-recombinant Der f 2 monoclonal antibody as the first antibody. They were then reacted with the fluorescent isothiocyanate conjugated goat anti-mouse IgG as the secondary antibody. The sections were examined with fluorescent light microscopy. RESULTS: Der f 2 reacting with immunofluorescent antibodies was found localized in the tissue and contents of the mid-gut of the mite. CONCLUSION: The major allergen Der f 2 distributes in the gut and fecal pellets of Dermatophagoides farinae.
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