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  • Title: IPG with electrodic plateaus (and other unusual procedures for 2-DE).
    Author: Gianazza E, Rocco AG, Marchetto A, Vergani L.
    Journal: Electrophoresis; 2007 Aug; 28(16):2953-6. PubMed ID: 17640092.
    Abstract:
    We describe some simple changes to the geometry of the IPG strips that make them suitable to the loading of very large sample volumes and of high-salt solutions. Of special relevance is the possibility of using strips with immobilized plateau(s) to either side of the gradient, or to both, also in connection with in-gel rehydration protocols and focusing in stock trays. The only requirement to achieve this is to leave the all-ready-made attitude and go back to custom polymerization of the IPGs in one's laboratory.
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