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  • Title: Propofol attenuates oxidative stress-induced PC12 cell injury via p38 MAP kinase dependent pathway.
    Author: Wu XJ, Zheng YJ, Cui YY, Zhu L, Lu Y, Chen HZ.
    Journal: Acta Pharmacol Sin; 2007 Aug; 28(8):1123-8. PubMed ID: 17640472.
    Abstract:
    AIM: To investigate the neuroprotective effect of propofol and its intracellular mechanism on neurons in vitro. METHODS: Cell viability was determined with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide reduction. Apoptotic cell death was determined by Hoechst 33258 staining and a fluorescence-activated cell sorter. The caspase-3 activity was measured by fluorometric assay. Mitogen-activated protein (MAP) kinase phosphorylation was detected with Western blotting. RESULTS: The pretreatment of rat pheochromocytoma cell line PC12 with propofol (1-10 micromol/L) resulted in a significant recovery from hydrogen peroxide (H2O2)-induced cell death and the inhibition of H2O2 induced caspase-3 activation and PC12 cell apoptosis. Propofol inhibited the H2O2-induced p38 MAP kinase, but not c-Jun N-terminal kinase or extracellular signal-regulated kinase 1 and 2 activations. CONCLUSION: Propofol might attenuate H2O2-induced PC12 cell death through the inhibition of signaling pathways mediated by the p38 MAP kinase.
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