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  • Title: IL-4-induced AID expression and its relevance to IgA class switch recombination.
    Author: Kim RJ, Kim HA, Park JB, Park SR, Jeon SH, Seo GY, Seo DW, Seo SR, Chun GT, Kim NS, Yie SW, Byeon WH, Kim PH.
    Journal: Biochem Biophys Res Commun; 2007 Sep 21; 361(2):398-403. PubMed ID: 17645870.
    Abstract:
    Activation-induced cytidine deaminase (AID) is an inducible gene that plays a critical role in Ig class switch recombination and somatic hypermutation in B cells. We explored the mechanisms by which IL-4 induces AID expression in mouse B cells. IL-4 increased AID expression and over-expression of Stat6 further augmented IL-4-induced promoter activity. The involvement of Stat6 in the promoter activity was confirmed using ChIP assays and site-directed mutagenesis. Treatment with H89, a PKA inhibitor, markedly decreased IL-4-induced AID expression, and over-expression of CREB enhanced it. These results indicate that Stat6 and PKA/CREB are involved in IL-4-induced AID expression. The relevance of these signal transducing molecules was verified using the TGFbeta1-induced IgA isotype switching model. Our results indicate that IL-4, through Stat6 and PKA/CREB, induces AID expression leading to Ig isotype switching event.
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