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  • Title: [Preparation and evaluation of a time-resolved fluoroimmunoassay kit for CA50 determination].
    Author: Hang JF, Wu YS, Xu WW, Yu WH, Huang Y, Li M.
    Journal: Zhonghua Zhong Liu Za Zhi; 2007 Mar; 29(3):189-92. PubMed ID: 17649634.
    Abstract:
    OBJECTIVE: To prepare a rapid and sensitive diagnostic kit for detection of CA50 based on time-resolved fluoroimmunoassay. METHODS: A sandwich-TRFIA diagmostic kit was developed using anti-CA50 monoclonal antibody and all parameters of the kit were evaluated. RESULTS: The linear measurement range of the kit was (5 - 300) U/ml. The sensitivity was 0.2 U/ml. The intra- and inter-assay coefficients of variation were 4.3% - 8.2% and 7.7% - 11.2%, respectively. There was no cross-reaction with CEA, CA12-5, CA15-3 and AFP. The cross reactivity with CA19-9 was 0.7 U/ml. The correlation coefficient of detection results of 107 blood samples between this newly developed kit and commercially available CA50 RIA kit was 0.901. CONCLUSION: This newly developed CA50-TRFIA kit is a valuable test tool for clinical application with even better sensitivity, specificity and accuracy.
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