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  • Title: [Fluorescent proteins: physical-chemical properties and application in cell biology].
    Author: Stepanenko OV, Verkhusha VV, Kuznetsova IM, Turoverov KK.
    Journal: Tsitologiia; 2007; 49(5):395-420. PubMed ID: 17654827.
    Abstract:
    Green fluorescent protein (GFP) from jellyfish Aequorea victoria is the most extensively studied and widely used in cell biology protein. At present novel naturally occurring GFP-like proteins have been discovered and enhanced mutants of Aequorea GFP have been created. These mutants differ from wild-type GFP by stability, value of quantum yield, absorption and fluorescence spectra position and photochemical properties. GFP-like proteins are the fast growing family. This review is an attempt to characterize the main groups of GFP-like proteins, describe their structure and mechanisms of chromophore formation and summarize the main trends of their utilization as markers and biosensors in cell and molecular biology.
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