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  • Title: A novel vector element providing multicopy vector integration in Arxula adeninivorans.
    Author: Steinborn G, Gellissen G, Kunze G.
    Journal: FEMS Yeast Res; 2007 Oct; 7(7):1197-205. PubMed ID: 17655689.
    Abstract:
    An Arxula adeninivorans vector element has been identified that provides multicopy integration in an atrp1 host strain. The element consists of the ATRP1 selection marker fused to a newly generated truncated ALEU2 promoter of 53 bp. In the described example eight copies of an amyA expression vector encoding heterologous alpha-amylase from Bacillus amyloliquefaciens are integrated in the genome of the recombinant strain instead of a single copy observed when using the ATRP1 element with the complete promoter. The high copy number results in strains of superior productivity for a secreted recombinant alpha-amylase. The vector design enables the integration of a small vector fragment that consists of yeast DNA only providing high transformation frequencies and a high mitotic stability.
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