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  • Title: Rapid and automated enumeration of viable bacteria in compost using a micro-colony auto counting system.
    Author: Wang X, Yamaguchi N, Someya T, Nasu M.
    Journal: J Microbiol Methods; 2007 Oct; 71(1):1-6. PubMed ID: 17669529.
    Abstract:
    The micro-colony method was used to enumerate viable bacteria in composts. Cells were vacuum-filtered onto polycarbonate filters and incubated for 18 h on LB medium at 37 degrees C. Bacteria on the filters were stained with SYBR Green II, and enumerated using a newly developed micro-colony auto counting system which can automatically count micro-colonies on half the area of the filter within 90 s. A large number of bacteria in samples retained physiological activity and formed micro-colonies within 18 h, whereas most could not form large colonies on conventional media within 1 week. The results showed that this convenient technique can enumerate viable bacteria in compost rapidly for its efficient quality control.
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