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  • Title: Targeted high-throughput sequencing of tagged nucleic acid samples.
    Author: Meyer M, Stenzel U, Myles S, Prüfer K, Hofreiter M.
    Journal: Nucleic Acids Res; 2007; 35(15):e97. PubMed ID: 17670798.
    Abstract:
    High-throughput 454 DNA sequencing technology allows much faster and more cost-effective sequencing than traditional Sanger sequencing. However, the technology imposes inherent limitations on the number of samples that can be processed in parallel. Here we introduce parallel tagged sequencing (PTS), a simple, inexpensive and flexible barcoding technique that can be used for parallel sequencing any number and type of double-stranded nucleic acid samples. We demonstrate that PTS is particularly powerful for sequencing contiguous DNA fragments such as mtDNA genomes: in theory as many as 250 mammalian mtDNA genomes can be sequenced in a single GS FLX run. PTS dramatically increases the sequencing throughput of samples in parallel and thus fully mobilizes the resources of the 454 technology for targeted sequencing.
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