These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Effect of 6-hydroxydopamine on the levels of phosphorylation of signal transduction and transcription factor 1 and 3: experiment with dopaminergic neurons].
    Author: Wang LL, Chen B, Xu SL.
    Journal: Zhonghua Yi Xue Za Zhi; 2007 Apr 17; 87(15):1048-52. PubMed ID: 17672970.
    Abstract:
    OBJECTIVE: To observe the effects of 6-hydroxydopamine (6-OHDA) on the levels of phosphorylation of signal transducer and activator of transcription (STAT) 1 and STAT3, and the role of STASD in the neurotoxicity of 6-OHDA on dopaminergic neurons so as to help study the treatment of Parkinson's disease. METHODS: Dopaminergic neurons, human neuroblastoma cells of the line SH-SY5Y were cultured and were treated with 6-OHDA of the concentrations of 50, 100, 200, and 400 micromol/L respectively. SH-SY5Y cells not treated with 6-OHDA were used as control group. MTT method was used to observe the cell viability rate 24, 48, and 72 h later. 3H-thymidine deoxyribose (TdR) was added into the culture fluid 12, 36, or 60 h after the treatment of 6-OHDA for 12 h, then the cell viability rate was observed. Western blotting was used to detect the levels of STAT1 and STAT3 phosphorylation. RESULTS: Twenty-four hours after the treatment of 50 - 400 micromol/l 6-OHDA the cell viability rates decreased to 8.1% - 81.9% that of the control cells (all P < 0.05 or P < 0.01), e.g., 100 micromol/L 6-OHDA decreased the cell viability rate to 50% that of the control cells (P < 0.01). 3H-TdR proliferation assay showed that the cell viability rate decreased with the increase of the 6-OHDA concentration and time of treatment too. Western blotting showed that 0.5 - 24 h after the treatment with 100 micromol/L 6-OHDA, the STAT3 phosphorylation level was decreased obviously with the comparative band density of 0.747 +/- 0.011 - 0.238 +/- 0.007, and 2 hours after the treatment with 50 - 200 micromol/L 6-OHDA the comparative band density of STAT3 phosphorylation was 0.895 +/- 0.003 - 0.633 +/- 0.002 (all P < 0.05). However, the level of STAT1 phosphorylation was not influenced by the treatment of 6-OHDA of the concentration of 100 mol/L at different time points. CONCLUSIONS: Decreased STAT3 phosphorylation may be involved in the neurotoxicity mechanisms of 6-OHDA on dopaminergic neurons.
    [Abstract] [Full Text] [Related] [New Search]