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  • Title: [Content determination of bergenin in Ardisia pusilla by HPLC].
    Author: Song LK, Zhou WM.
    Journal: Zhong Yao Cai; 2007 Apr; 30(4):422-3. PubMed ID: 17674794.
    Abstract:
    OBJECTIVE: To determine the content of bergenin in Ardisia pusilla. METHODS: High Performance Liquid Chromatography was performed on Hyersil C18ODS. The chromatorgraphic conditions were as follows: methanol-water (20:80) as mobile phase, flow rate being 0.600 ml/min and detecting wavelength at 275 nm. The column temperature was 25 degrees C. RESULTS: A good linearity of bergenin was shown in range of 0. 098-0. 591 microg (r = 0.9998), the average recovery is 102.0%, RSD = 1.4% (n=6). The content of bergenin in Ardisia pusilla is 59% at least. CONCLUSION: The content of bergenin in Ardisia pusilla and A. japonica is identical.
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