These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Alterations in the glutathione-redox balance induced by the bio-insecticide Spinosad in CHO-K1 and Vero cells.
    Author: Pérez-Pertejo Y, Reguera RM, Ordóñez D, Balaña-Fouce R.
    Journal: Ecotoxicol Environ Saf; 2008 Jun; 70(2):251-8. PubMed ID: 17675233.
    Abstract:
    The biopesticide Spinosad causes a drop in cell viability in two mammalian cellular models CHO-K1 and Vero, using the neutral red incorporation assay as endpoint. Dose-response curves were assessed after 24, 48, and 72 h under different conditions i.e. presence of 10% fetal calf serum or 1% bovine serum albumin or antioxidants. The presence of antioxidant agents, viz. reduced glutathione (1 mM), vitamin C (100 microM), and vitamin E (20 microM) reduced significantly the cytotoxic effect of Spinosad, thus pointing to an oxidative damage mediated by this compound. An increase in malondialdehyde production was observed after 24-h treatment with Spinosad in both Vero and CHO-K1 cells, using fractions of NRU(50) as final concentrations. At concentrations equivalent to its NRU(20), NRU(10) and NRU(5) Spinosad caused significant alterations in the glutathione-redox cycle in the form of significant decrease in total and reduced glutathione, large increase in glutathione peroxidase activity, little induction of glutathione reductase, and significant decline of glutathione S-transferase activities.
    [Abstract] [Full Text] [Related] [New Search]