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  • Title: A semicontinuous 3-column countercurrent solvent gradient purification (MCSGP) process.
    Author: Aumann L, Morbidelli M.
    Journal: Biotechnol Bioeng; 2008 Feb 15; 99(3):728-33. PubMed ID: 17680681.
    Abstract:
    The recently developed continuous Multicolumn Countercurrent Solvent Gradient Purification (MCSGP) Process has been reduced to a fully equivalent semicontinuous setup with only three chromatographic columns and three gradient pump modules. Actually the 3-column MCSGP unit can even achieve better performance than the original 6-column process due to an additional degree of freedom, that is a different switching time for the "batch lane" and the "interconnected lane." Experimental results for the 3-column MCSGP unit of the purification of an industrial multicomponent peptide mixture containing 46% of Calcitonin on a reversed phase resin are compared with model simulations. It is concluded, that the model is well suited to predict the system behavior and therefore to design its optimal operating conditions.
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