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  • Title: Identification of less-common Streptococcus pneumoniae serotypes by a multiplex PCR-based reverse line blot hybridization assay.
    Author: Zhou F, Kong F, Tong Z, Gilbert GL.
    Journal: J Clin Microbiol; 2007 Oct; 45(10):3411-5. PubMed ID: 17687009.
    Abstract:
    We developed a multiplex PCR-based reverse line blot (mPCR/RLB) assay to identify 50 uncommon pneumococcal serotypes. In combination with a previously described mPCR/RLB assay (3), all 90 pneumococcal serotypes can be identified individually (32 serotypes) or, because of predictable cross-reactions, to within small groups of two to five related serotypes (58 serotypes), which can be distinguished using serotype-specific antisera.
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