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  • Title: Cell cycle and apoptosis alteration of human hepatocarcinoma cells by subclinical-dose 12C6+-beam irradiation.
    Author: Liu B, Zhang H, Xie Y, Hao J, Duan X, Zhou Q, Qiu R, Zhou G.
    Journal: Eur J Gastroenterol Hepatol; 2007 Sep; 19(9):749-54. PubMed ID: 17700259.
    Abstract:
    OBJECTIVE: The purpose of this study is to investigate the effect of subclinical-dose C-beam irradiation on cell cycle and cell apoptosis in hepatocarcinoma cells. MATERIALS AND METHODS: The HepG2 cells were exposed to 0-2.0 Gy of either the C beam or a gamma-ray. Cell survival was detected by clonogenic assay. Cell cycle was determined by flow-cytometry analysis. The apoptosis was monitored by fluorescence microscope with DAPI staining. p53 and p21 expression were detected by Western blot. RESULTS: The G0/G1 cells in the irradiated groups were significantly more than those in the control (P<0.05). The C-ion irradiation had a greater effect on the cell cycle of HepG2 cells (including promoting G1-phase and G2-phase arrest) than gamma-ray irradiation. The apoptotic cells induced by C beam were significantly more numerous than those induced by gamma-ray (P<0.05). The carbon ions had a stronger effect on p53 and p21 expression than the gamma-ray irradiation. The survival fractions for cells irradiated by C beam were significantly smaller than those irradiated by gamma-ray (P<0.05). CONCLUSION: The subclinical-dose C-beam irradiation significantly suppresses HepG2 cells through cell-cycle arrests and cell apoptosis in contrast to same-dose gamma-ray irradiation.
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