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  • Title: Combined enzymatic and chemical approaches to the synthesis of unique polyribonucleotides.
    Author: Broom AD, Uchic ME, Uchic JT.
    Journal: Biochim Biophys Acta; 1976 Mar 17; 425(3):278-86. PubMed ID: 177053.
    Abstract:
    The enzymatic polymerization by polynucleotide phosphorylase of 6-chloro-9-(beta-D-ribofuranosyl)purine 5'-diphosphate to poly(6-chloropurinylic acid) and its conversion to poly(6-thioninosinic acid) is described. The sulfur isostere of poly(I) was found not to form a complex with poly(C), but to form a self-association complex with a Tm around 295 degrees K. The sedimentation velocities, pKa and Tm values of the polymer have been examined under various conditions. A two (or more) stranded helical array is suggested as the most probable structure. Thermal loss of the thione chromophore was noted for poly- (S6I), S6IMP and S6I; the degradation product from S6I was shown to be inosine.
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