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Title: On the use of the spin labeling technique in the study of erythrocyte membranes. Author: Buttefield DA, Whisnant CC, Chesnut DB. Journal: Biochim Biophys Acta; 1976 Apr 05; 426(4):697-702. PubMed ID: 177059. Abstract: ESR spectra and scanning electron micrographs of human erythrocytes spin labeled with the conventional stearic acid nitroxide substituted at the 5-position have been obtained over a range of label-to-lipid ratios. While morphological changes as previously reported (Bieri, V. G., Wallach, D. F. H. and Lin, P. S. (1974) Proc. Natl. Acad. Sci. U.S. 71, 4797-4801) are reproduced, it is shown that at label-to-lipid ratios of 1:10 or less the basic ESR spectrum is not significantly affected. At low label concentrations the spin labeling technique is a viable one and can be used to investigate membrane properties.[Abstract] [Full Text] [Related] [New Search]